In this study, to better understand enhancer regulation in cancer, we show the epigenetic co-regulation of TMPRSS2 and PRCAT38 via eRNA transcription, enhancer-promoter interaction, and gene regulation by transcription factors, revealing a co-regulatory function of two enhancers, denoted by E1 and E2 in the transcription of PRCAT38 and TMPRSS2. In addition, the enhancer activity is mediated by AR/FOXA1 binding and the acetyltransferase p300. This evidence concerns the gene TMPRSS2 and cancer.