So, the current study elucidates for the first time the possible gastroprotective activity of RK against ethanol-induced acute gastric ulceration in rats, in addition, identify the role of HMGB1 in ethanol-induced gastric ulceration, and the crosstalk of Nrf2 with HMGB1 and NOXs. This evidence concerns the gene HMGB1 and gastric ulcer.