To further define the role of G3BP1 in the norovirus life cycle and to confirm that the function of G3BP1 was downstream of virus binding and viral entry, we therefore bypassed the entry phase of the infection process and transfected MNV VPg-linked RNA into WT and two independently generated BV2 ΔG3BP1 cell lines and examined the impact on norovirus replication. This evidence concerns the gene G3BP1 and infection.