IFNB1 and hepatocellular carcinoma: Notably, rt269I showed enhanced IFN-I, even compared to genotype A wild type with the same isoleucine at the 269th amino acid of RT at 24 h after transfection (Figure S4D), suggesting that the trait capable of IFN-I induction as found in rt269I may be specific to genotype C. In addition, the HBV encoding DNA was also transfected into mouse hepatoma cell Hepa1c1c-7 and IFN-β was measured assessed by ELISA (Figure S4E).