FOXO1 and nonpapillary renal cell carcinoma: Furthermore, FOXO1 expression at both the mRNA and protein levels were reduced by miR-27a-3p overexpression and increased by miR-27a-3p knockdown in ccRCC cells, as determined by qRT-PCR and western blot assays (Figure 6D, 6E, P<0.05, P<0.01).