To investigate the impact of ADAMTS9-AS2 or miR-27a-3p on ccRCC, cell proliferation was subsequently analyzed by 3-(4, 5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfop-henyl)-2H-tetrazolium (MTS) assay in both 786-O and caki-1 cells after ADAMTS9-AS2/miR-27a-3p overexpression or knockdown. The gene discussed is ADAMTS9; the disease is nonpapillary renal cell carcinoma.