Since no CD74-ROS1 rearrangement-harboring cancer cell line was publicly available, two CD74-ROS1 and one EZR-ROS1-rearranged cancer cells were established from the pleural effusions of three TKI-naive patients (JFCR-165, JFCR-168, and MGH-193-1). The gene discussed is ROS1; the disease is cancer.