HPN and posterior cortical atrophy: To assess whether increased autophagy is restricted to the PC-3 cell model or a general phenomenon following hepsin overexpression, we investigated LC3B-II abundance (i) by western blot analysis in stably transfected human embryonal kidney HEK293 cells providing inducible overexpression of wild-type hepsin, and (ii) by double immunofluorescence analysis (HPN/LC3B) in androgen-receptor (AR)-positive LNCaP PCa cells post transient overexpression of wild-type vs. protease-deficient hepsin.