To find molecular mechanism of the antitumor effects of THZ1 in CCA, we carried out RNA-Seq and found there were more than 1000 genes downregulated in three CCA cell lines after THZ1 treatment, including some well-known oncogenes such as SP1, FOSL1, RUNX1, and MCL1. This evidence concerns the gene RUNX1 and cholangiocarcinoma.