Kaplan–Meier analysis revealed that overall survival of patients was worse in the high-risk score group than in the low-risk score group (log-rank test: p = 0.005; Fig. 6b) indicating that the risk score model based on Notch4, p-PKCα/β2, XIAP, CDK2, and Akt1 expression predicts GC prognosis. The gene discussed is NOTCH4; the disease is gastric cancer.