Thereafter, KEGG pathway enrichment analysis using gene set enrichment analysis (GSEA) software suggested that PI3K/Akt signaling pathway was inactivated in periodontal tissues with periodontitis compared with healthy samples (Figure 1B, 1C), which was better visualized by joyplot and dotplot as shown in Figure 1D–1F. The gene discussed is AKT1; the disease is periodontitis.