NCAM1 and neoplasm: Defining NKT cells as CD3+CD56+γδTCR- (Fig 4A), at the end of initial expansion, 21.3±2.3% of CD3+ cells (n = 31 tumor fragments) had this phenotype compared with 37.8±3.6% following rapid expansion (n = 41 tumor fragments) representing a significant increase in NKT cell abundance (p = 0.0006 by unpaired t-test, Fig 4B).