Indeed, ESAT-6 suppresses MΦs to IFN-γ,25–27 and induces suppressive IL-6 and TGF-β through TLR-2 signaling, thereby affecting cytosolic translocation of Mtb.93,94 Therefore during tuberculosis pathogenesis, ESAT-6 and CFP-10 complex perhaps skews immune responses beneficial to the pathogen.27 On the other hand, CFP-10 elicits robust response among humans and contained the autophagy-inducing and TLR-2 activating C5 motif.95 By expressing the C5 immunogenic moiety of Mtb in BCG85B, we markedly improved BCG vaccine as outlined below. This evidence concerns the gene TGFB1 and tuberculosis.