As shown in Figure 3B, except for OPG the mRNA expression of IL-6, IL-8, IL-1β, and RANKL in RA-FLSs up-regulated more or less after induced by TNF-α(10 ng/mL), but DIM(25 and 50 μM) inhibited IL-6, IL-8, and IL-1β mRNA expression levels increase caused by TNF-α(10 ng/mL) and had no significant effect to RANKL. Neither TNF-α nor DIM treatment obviously altered IL-17 mRNA expression. This evidence concerns the gene CXCL8 and rheumatoid arthritis.