To confirm the effect of ANLN knockdown on the expression of EZH2 and LASP1, we detected the levels of ANLN, EZH2 and LASP1 expression in tissues from in vivo xenograft tumor models established with the stable ANLN-silenced BxPC-3 cells or the stable scramble control BxPC-3 cells by IHC and Western blot. The gene discussed is EZH2; the disease is neoplasm.