Consistent with previous reports, our results from the GENT database and IHC analysis showed that ANLN expression was significantly upregulated in pancreatic cancer tissues, and increased ANLN expression was significantly correlated with the tumor size, tumor differentiation, TNM stage, lymph node metastasis, distant metastasis and poorer outcome of patients with pancreatic cancer. This evidence concerns the gene ANLN and familial pancreatic carcinoma.