IFNA1 and neoplasm: Instead of fusing mIFN-α to the N terminal of a human IgG Fc, we fused mIFN-α to the C terminal of a mouse IgG heavy chain (Fig 1A and 1B) and this approach has been shown to significantly decrease the IFN-α activity in a control fusion that does not engage tumor antigen [4], thereby limiting the potential off target toxicity of the fusion protein.