In view of STAT3 signaling pathway is constitutively activated in cholangiocarcinoma and closely associated with proliferation, apoptosis and metastasis of tumor cells, we then investigated the effect of Huaier and 5-FU alone or their combination on STAT3 and its activation form p-STAT3(tyrosine-phosphorylated STAT3. The gene discussed is STAT3; the disease is cholangiocarcinoma.