In 2014, Perez-Carbonell and colleagues evaluated the methylation status of Septin 9 (SEPT9), Aristaless-Like Homeobox 4 (ALX4), Twist-related protein 1 (TWIST1), Insulin Like Growth Factor Binding Protein 3 (IGFBP3), Growth Arrest Specific 7 (GAS7), and miR137 in CRC patients, and its association with clinicopathological characteristics [37]. The gene discussed is TWIST1; the disease is colorectal carcinoma.