ING4 and neoplasm: It was found that Ad-ING4 transfection led to a suppression of A549 tumor cell growth both in vitro and in vivo [47]: Cells were cultured either in the presence or absence of Ad-ING4, and the respective effects on tumor cell growth was monitored for the duration of four days via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid (MTT) assay.