For example, overexpression of LIMK1 kinase domain abolished pseudopodia extension and cell migration of rat metastatic mammary adenocarcinoma cell MTLn3.38 In mouse myoblast C2C12 cell, activation of PAK4 or LIMK1 decreased actin severance, caused aberrant polymerization of actin filaments and reduced pseudopodia protrusion, which was reversed by expressing constitutively activated cofilin.34 The gene discussed is PAK4; the disease is breast adenocarcinoma.