Additionally, since T cells activated in LNs can be further stimulated and either remain as CD8+ memory T cells or migrate to the tumour microenvironment [13, 53] and LNs are the most common sites of solid-tumour metastases [40], we further examined the biodistribution of these OT-I CD8+ T cells in different secondary lymphoid organs by analysing the presence of CD8+Vα2/Vβ5+ T cells in the tumour-draining LN, the primary site of metastasis initiation, as well as a LN distal to the tumour and the spleen. Here, CD8A is linked to neoplasm.