An analysis of the activation of these cells in the different groups showed that, in general, the cells that had infiltrated the tumours of all the groups treated with OT-I CD8+ T cells seemed to present a higher activation state (i.e., higher percentage of CD69+ and CD25+ cells) and to produce more IFN-γ after restimulation with the OVA257–264 peptide. This evidence concerns the gene CD8A and neoplasm.