In this study, we wanted to see the effectiveness of nicotine as a preventive agent for osteoporosis by examining bone turnover marker: serum osteocalcin, serum deoxypyridinoline (DPD) and serum calcitonin in ovariectomized wistar strain rats as model of postmenopausal osteoporosis. This evidence concerns the gene BGLAP and postmenopausal osteoporosis.