To test this hypothesis, we overexpressed TXNRD1 in HCC cells transfected with the miR-125b-5p mimic (Fig. 5a, b), and found that TXNRD1 overexpression reversed miR-125b-5p-mediated suppression of HCC cell proliferation, migration, and invasion according to EdU-incorporation, Transwell, and Boyden assays, respectively (Fig. 5c–h). This evidence concerns the gene TXNRD1 and hepatocellular carcinoma.