CD274 and non-small cell lung carcinoma: For example, to identify patients with non-small cell lung cancer for second line therapy with pembrolizumab where PD-L1 IHC pharmDx 22C3 is not available, the results of our study indicate that it is more likely that 22C3 or E1L3N well-developed, fit-for-purpose laboratory developed tests would identify the same patients as positive and/or negative as PD-L1 IHC pharmDx 22C3, rather than Ventana PD-L1 (SP263), Ventana PD-L1 (SP142), or PD-L1 IHC pharmDx 28-8, which were developed for different purposes [49–53].