Of note, although knockdown efficiencies were variable between cell lines (Fig. S2B) and did not persist over the full time course of tumor growth, increased p62 staining was observed in ATG5- and RAB21-depleted CRC tumors, suggesting that autophagy was impaired, in our CAM model (Fig. S4D to G). The gene discussed is ATG5; the disease is colorectal carcinoma.