The third lncRNA most upregulated in primary melanoma cell lines in comparison to melanocytes was ZEB1-AS1, which is located in the opposite strand of gene ZEB1. Importantly, the lncRNA ZEB1-AS1 was also amongst the top five lncRNAs upregulated in metastatic melanoma cell lines when compared to melanocytes. This evidence concerns the gene ZEB1 and metastatic melanoma.