Collectively, based on analysis of NEC miRNA and gene expression chips and the construction of differentially expressed miRNA-DEG-disease gene regulatory network, our study provided evidence that miR-429/200a/b and miR-141/200c clusters might serve as important biomarkers for NEC in newborn by binding to VEGFA, SELE, KDR, FLT1, and HGF. Here, SELE is linked to necrotizing enterocolitis.