We opted to isolate from this milieu two categories representing extreme ends of the continuum: (1) those with no TP53 mutation or only a subclonal mutation, which we hypothesized to have low risk-of-relapse, and (2) inversely, those that had a mutation and some other compounding event such as loss-of-heterozygosity or 17p copy loss, which we called “high-risk.” We additionally hypothesized the risk-of-relapse for all other cancers would be “moderate.” Of note, we set the normal reference range for allele frequency at 38–59% based upon a confidence interval obtained from normal samples. Here, TP53 is linked to cancer.