In prostate cancer metastases (Fig. 2b) significant hypermethylation of one EBF3 (cg03774288) gene body CpG and four TBC1D16 (cg07618085, cg17295878, cg23651872, cg19004465) CpG sites (both + 16%) were observed compared to normal prostate tissue (red vs blue boxplots). This evidence concerns the gene TBC1D16 and Familial prostate cancer.