EBF3 and metastatic melanoma: In previous genome-wide DNA methylation analysis of three cutaneous primary and metastatic melanoma cell line pairs using reduced representation bisulfite sequencing (RRBS), we showed that an RRBS fragment in the promoter of EBF3 (at chr10:131763530–131763587, GRCh37) was significantly hypermethylated in metastatic cell lines compared to matched primary cell lines [9].