To evaluate DNA methylation changes in both the promoter and gene body of EBF3 and TBC1D16, we interrogated all analysed CpG sites in independent whole genome bisulfite sequencing (WGBS) data [18] derived from five different normal tissues/cell lines (B cells, lung, brain, breast and colon), six different primary tumours/cell lines (small cell lung carcinoma, squamous cell carcinoma of the lung, adenocarcinoma of the lung, glioma, primary breast cancer, primary colorectal cancer) and metastases (breast, colorectal) (Fig. 4). The gene discussed is TBC1D16; the disease is central nervous system cancer.