Almost all analysed CpG sites in EBF3 and TBC1D16 were significantly differentially methylated (− 14% in the gene body of EBF3 and + 9% in the gene body of TBC1D16) in both adenomas and carcinomas compared to normal colon tissue (red vs blue and green vs blue boxplots, respectively). The gene discussed is EBF3; the disease is adenoma.