TBC1D16 and endometrial cancer: In endometrial cancer (Fig. 2a), CpG sites in the gene bodies of EBF3 (cg09649486, cg25866634) and TBC1D16 (cg07618085) were significantly differentially methylated (+ 17% and − 14%, respectively) in lymph node metastases compared to primary tumours (green vs red boxplots).