TBC1D16 and melanoma: To investigate whether the findings from melanoma were generalisable to changes in methylation occurring during tumourigenesis or metastasis in multiple other cancer types, we next investigated previously published publicly available data from 450k methylation analysis of tissues of endometrial cancer (Fig. 2a), prostate cancer (Fig. 2b) and triple-negative breast cancer (Fig. 2c) to identify differences in methylation of EBF3 and TBC1D16 gene body and promoter regions.