Meanwhile, a western blot assay was used to measure the Notch1 expression in GBM cell lines, the results revealed that Notch1 was commonly expressed in GBM cell lines (LN-18, SNB-19, U87, U251, LNZ308), highly expressed in the U87, U251 and LN-18 cell lines (Additional file 1: Figure S1b). Here, NOTCH1 is linked to glioblastoma.