Western blot analysis of different grades of glioma tissues also demonstrated that Notch1 is highly expressed in high grade gliomas (WHO III-IV) (Additional file 1: Figure S1a). In addition, the expression profile of Hes1 and its Kaplan-Meier analysis also confirmed this finding (Additional file 2: Figure S2a-e). The gene discussed is NOTCH1; the disease is central nervous system cancer.