Figure 5b (3.14 vs 0.95) and c (2.1 or 2.4 vs 1) also reveal that, as the target gene locus of mir-322 in mice and the most likely target gene locus of mir-424 in the seminal plasma of male infertility patients with high DFI, Ddx3x showed a significant increase in mRNA (3.14 vs 0.95) and protein (2.1 or 2.4 vs 1) levels (*P < 0.05). The gene discussed is DDX3X; the disease is male infertility.