One of the most important findings of this study is that the methylation level of the RELA gene showed positive correlation with NF-κB1 transcripts depending on expression of TNF-α in tumor tissues, whereas the methylation status of the NFKB1 and the RELA genes were not directly associated with the level of NF-κB p50 transcripts. The gene discussed is NFKB1; the disease is neoplasm.