AGTR1 and osteosarcoma: Furthermore, Nakai et al. [120] showed that angiotensin II acting via the AT1 receptor led to phosphorylated ERK1/2, which induced MMP-3 and MMP-13 gene expression by the rat osteosarcoma cell line ROS17/2.8, a finding that was completely blocked when the ERK inhibitor, PD98059, or losartan, a selective competitive angiotensin II receptor type 1 antagonist, was added.