To confirm that interphasic H3 phosphorylation is necessary for gene expression activation, we investigated whether the silencing, or inhibition, of RSK2 affects a subset of genes (Aurora kinase B, AURKB; Kinesin-like protein KIF23, KIF23; Chromosome-associated kinesin KIF4A, KIF4A; Centromere protein F, CENPF) belonging to the HMGA1 signature that we previously identified in BC cells [29] and from now on referred to as the HMGA1 subset signature (Hsss). The gene discussed is CENPF; the disease is breast cancer.