Regarding the downstream effects of PKC isoforms on ERM proteins, recombinant human PKCα can phosphorylate ezrin in a cell-free assay in a Ca2+ and phospholipid dependent manner, and PKCα can phosphorylate the ERM C-terminal threonine residue within a kinase–ezrin molecular complex, in breast cancer epithelial cells [55]. The gene discussed is PRKCA; the disease is breast carcinoma.