These studies corroborate previous findings related to the mechanisms of NOX-derived ROS production under hyperglycemia; high glucose-mediated activation of NOX are attributed to diacylglycerol (DAG) and PKC signaling by promoting phosphorylation of the subunits p47phox, p67phox, and p22phox [138,145]. This evidence concerns the gene CYBA and Hyperglycemia.