NFKB1 and lentivirus infection: To confirm the role of EZH2 in NF-κB methylation, EZH2/NF-κB complex was pulled down with NF-κB antibody and immunoblotted with Methyl K. GSCs following shEZH2 carrying lentivirus infection or treatment of DZNep, presented significantly lower level of Methyl K compared to uninfected/untreated controls (Figures 4C,D).