Moreover, RNAi-mediated depletion of Jagged1 and Jagged2 proteins in ErbB2-negative breast cancer cell lines inhibited proliferation and induced apoptosis in vitro, thus demonstrating an important autocrine/juxtacrine loop between Jagged1/Jagged2 ligands and Notch3 in TNBC context [99], which was then also observed in other tumor contexts [100]. This evidence concerns the gene JAG1 and breast carcinoma.