Figure 4(f) shows expression of ABCB5 primarily in CD133(+), but not CD133(-) cells. The ability to form spheroids is a hallmark of cancer-initiating cells. DsRed CD133(+) and GFP CD133(-) subpopulations were separated by MACS. CD133(+) cells, but not CD133(-) cells, formed large melanospheres on poly-HEMA (Figure 4(g)). This evidence concerns the gene ABCB5 and cancer.