TNF and neuroblastoma: Although a previous study reported that Tnf-α treatment results in NFAT nuclear translocation via activation of calcineurin in the neuroblastoma cells (33), in our experiments Nfatc4 nuclear translocation was not blocked by Tnf inhibition using Len (Figure S3), suggesting that Nfatc4 works upstream of Tnf in neomycin-induced hair cell apoptosis.