Eighteen PD-L1 positive regions where the HCC cells expressed PD-L1 protein (≥10 PD-L1+ cells / high power field) and eighteen PD-L1 negative regions where the PD-L1 expression is negative or low (<10 PD-L1+ cells / high power field) were selected from the PD-L1 positive cases. This evidence concerns the gene CD274 and hepatocellular carcinoma.