As illustrated in Figure 4A, consumption of a HFD resulted in an abnormal appearance with atypical yellowish coloration of the livers from WT mice with severe steatosis and lipid droplet accumulation, while livers from Nlrp3−/− and Asc−/− (Pycard−/−) mice retained dark red coloration without steatosis. This evidence concerns the gene PYCARD and steatosis.