To explore the role of HSP90α/β and β as potential biomarkers in adrenal tumors, their expression was evaluated by immunohistochemistry in FFPE samples from patient group 1, which included 32 patients with non-functional adenomas (NFA), adenomas associated with autonomous cortisol secretion (ACS) and overt Cushing's syndrome (CS) and ACC, respectively (Supplemental Table 1A). This evidence concerns the gene HSP90AA1 and Cowden syndrome 1.