Similar to recent findings in pituitary cells (39), treatment of adrenocortical cancer cell lines with N-terminal HSP90 inhibitors decreased their glucocorticoid receptor expression and additionally we observed a reduction of cortisol secretion in NCI-H295R cells upon C-terminal and N-terminal HSP90 inhibitor treatment at time points without relevant effects on cell viability. The gene discussed is NR3C1; the disease is adrenal cortex carcinoma.