To further confirm the accuracy of high throughput data, bisulfite-pyrosequencing was performed on Chr.16:28856729, Chr.16:28856735, and Chr.16:28856743 located at SH2B1 in 30 pairs of sporadic ADOS-diagnosis autism, in comparison to the respective age- and sex-matched controls (see Supplementary Table 4). The gene discussed is SH2B1; the disease is autism.