To explore whether Ser469, Ser495, and Ser533 of ULK1 phosphorylated by TOPK affects the sensitivity of glioma cells to TMZ, Hs683 and H4 cells were transfected with ULK1-WT or ULK1-AAA to establish cell lines stably expressing ULK1 wild type and mutant. This evidence concerns the gene ULK1 and central nervous system cancer.