Then autophagy was inhibited by wortmannin, the expression of Cleaved-Caspase-3 in each group was decreased, but its expression in ULK1-AAA cells was still significantly higher than that in ULK1-WT cells (Fig. 7a), indicating that the mutation of ULK1 promoted apoptosis. The gene discussed is ULK1; the disease is triple-A syndrome.