KLF4 wasdown-regulated in both tumor samples and mammospheres.Meanwhile, expression of NANOG was not changed inmammospheres, but it was down-regulated in tumors (Fig .3B).Among EMT-related genes, CDH2, SNAIL1, TWIST1/2 andZEB1 were also overexpressed in mammospheres. The gene discussed is TWIST1; the disease is neoplasm.