In the next step, expression level of stemness relatedgenes (OCT4, SOX2, KLF4, c-MYC and NANOG) and EMTtranscription factors (CDH1, CDH2, SNAIL1, TWIST1,TWIST2 and ZEB1) were evaluated in all tumor samples andmammospheres. This evidence concerns the gene TWIST1 and neoplasm.