Overall, we found our results to indicate that on average (and for individual cases) anti-PD-1 administered first followed by anti-CTLA-4 (Fig. 5A, blue) produced a greater effect on tumor response than anti-CTLA-4 first followed by anti-PD-1 (Fig. 5A, red), consistent with clinical results. The gene discussed is PDCD1; the disease is neoplasm.