SLC9A1 and breast carcinoma: As expected from in situ proximity ligation assays (PLA), which confirmed the close proximity (<40 nm) of endogenous NHE1 with endogenous CN in MCF-7 breast cancer cells (Fig. 2i, j), stably expressed WT NHE1 robustly co-immunoprecipitated with CN in PS120 cells, with and without an increase in cellular [Ca2+]i (Fig. 2h, Supplementary Fig. 2).