PIK3CA and cervical squamous intraepithelial neoplasia: We recently reported that expression of the PIK3CAH1047R mutant can lead to centrosome amplification (in mouse embryonic fibroblasts (MEFs), the MCF10A immortalised breast cell line and in mouse tissues) and increased in vitro tolerance to WGD (in MEFs) [93], indicating that PI3K activation might be involved in CIN (Figure 2B).