Moreover, increased methylation was positively correlated with high density lipoprotein (HDL) concentration in blood and was decreased in muscle tissue from diabetic patients.147 This relationship with HDL concentration was also reproduced independently in an EWAS focused on serum lipid profiles conducted in a separate cohort.149 Relatedly, Wu and colleagues150 further found an association between PHOSPHO1 and SNPs relating to clinical measures of obesity in a Chinese population. This evidence concerns the gene PHOSPHO1 and obesity due to melanocortin 4 receptor deficiency.