CSCs are a small population of cells responsible for initiation of tumor burden and therapeutic refractoriness.27 Acquisition of self‐renewal capacity and stemness properties at the time of tumorigenesis is an essential prerequisite for further accumulation of oncogenic transformations and eventual development of cancer.28 Here, we sought to identify CSC‐like cells by inducing ectopic overexpression of CD133 in two PC cell lines (PC3 and DU145). The gene discussed is PROM1; the disease is pachyonychia congenita.